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Identification, purification and quantification of Toxins “Ochratoxin A” in Algerian grape juice
ISSN: 2716 - 9278
Publisher: author
Identification, purification and quantification of Toxins “Ochratoxin A” in Algerian grape juice
Indexed in
Technology and Engineering
ARTICLE-FACTOR
1.3
Article Basics Score: 3
Article Transparency Score: 3
Article Operation Score: 3
Article Articles Score: 3
Article Accessibility Score: 3
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-1802
Publisher: Identification, Purification And Quantification Of Toxins ..
International Journal Address (IAA):
IAA.ZONE/271689059278
eISSN
:
2716 - 9278
VALID
ISSN Validator
Abstract
This study aimed to investigate the mycotoxin of Ochratoxin A (OTA) which can contaminate grape in the Mediterranean basin. Its toxicity was the subject of many animal studies and carcinogenic characters are well established today. So the European Committee has fixed the maximum in grape 2µg/L. Most methods used for mycologic identification is based mainly on the morphological characters. It was identified after its purification by the technique of culture monospore on the culture medias Potatoes Agar and Czapek Yeast extract Agar. The detection and quantification of this mycotoxin by uses combined techniques: immunoaffinity for the purification and the HPLC coupled with the fluorometric detector. This chromatographic system allowed us to estimate the contamination of Ochratoxin in the grape which is lower than 30 ng/L. The concentration of conidia at Aspergillus section Nigri was estimated at 3×106conidia/mL. the value obtained is le...
