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An Efficient Protocol for Long-Term Preservation of Cyanobacteria
ISSN: 0976 - 7614
Publisher: author
An Efficient Protocol for Long-Term Preservation of Cyanobacteria
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 3
Article Operation Score: 2
Article Articles Score: 2
Article Accessibility Score: 3
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097653697614
eISSN
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0976 - 7614
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Abstract
A simple modification in preparation of nutrient agar meant for agar slants resulted in a convenient and efficient matrix for long-term cyanobacterial preservation. In the modified protocol, agar concentration was increased and cyanobacterial cells were mixed rapidly in the molten agar before solidification. Solidified mixture was cut into cubes and air dried. The resulting agar flakes were stored in the dark. Periodically some flakes were inoculated in fresh medium to study regeneration of viable filaments in order to access the efficiency of the method in preserving cyanobacteria in dehydrated form. Possible outer contamination could be removed by washing the agar flakes in 1% sodium hypochlorite for one minute prior to their inoculation in fresh medium. The percentage of agar used and amount of cyanobacterial cells entrapped were the factors that influenced the period of preservation. A comparison of some biochemical and physiologi...
