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Standardization of free radical scavenging activity of some indigenous medicinal plants by using thin layer chromatogr...
ISSN: 0976 - 7614
Publisher: author
Standardization of free radical scavenging activity of some indigenous medicinal plants by using thin layer chromatogr...
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 2
Article Operation Score: 3
Article Articles Score: 2
Article Accessibility Score: 2
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097652187614
eISSN
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0976 - 7614
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Abstract
Thin layer chromatography is basically used to separate out plants active constituents, various dyes and chemical analysis. The present study is designed to find out free radical scavenging property with the help of thin layer chromatography. For conducting this experiment 15 medicinal plants of local region were selected. Collected plants were shade dried, grind to a coarse powder and subjected to extraction with Soxhlet apparatus followed by concentration under reduced pressure by rotatory evaporator. The Methanolic plant extract was dissolved in methanol and 1µl of extract of 50mg/ml concentration was spotted on the aluminum silica plate (1.05554.0007 spectrum, Merck Germany). It was then subjected to run in Toluene: ethyl acetate (70:20) solvent mobile phase. Run plate was removed, dried and calculated Rf values for every separated active constituent. The developed plate sprayed with DPPH (free radical scavenger) reagent at 0.1mM...
