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Evaluating the efficacy of a Plasmodium species-specific Multiplex-Nest-PCR in malaria diagnosis using different DNA i...
ISSN: 0976 - 7614
Publisher: author
Evaluating the efficacy of a Plasmodium species-specific Multiplex-Nest-PCR in malaria diagnosis using different DNA i...
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 3
Article Operation Score: 3
Article Articles Score: 2
Article Accessibility Score: 3
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097644987614
eISSN
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0976 - 7614
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Abstract
Malaria diagnosis and speciation still rely on microscopic identification in many settings. But, microscopy is tedious and lack sensitivity, particularly in areas under advanced eradication programs where low-density infections are increasingly reported. Species-specific molecular techniques are highly sensitive and reliable alternatives for Plasmodium parasites detection and speciation. Nevertheless, the efficacy of molecular techniques is directly affected by the purity and quality of isolated DNA templates. A Plasmodium species-specific multiplex-nested-PCR was assessed using DNA templates prepared separately by phenol-chloroform method, a DNA-precipitation commercial kit, and a chromatographic commercial kit from 126 EDTA-preserved whole blood samples. 115 samples were collected from malaria suspicious febrile patients in endemic southern region and 11 malaria positive samples from foreign and national visitors of non-endemic west...
