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Conventional and molecular differentiation between capsular types of Pasteurella multocida isolated from various anima...
ISSN: 0976 - 7614
Publisher: author
Conventional and molecular differentiation between capsular types of Pasteurella multocida isolated from various anima...
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Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 2
Article Operation Score: 3
Article Articles Score: 3
Article Accessibility Score: 3
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097644527614
eISSN
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0976 - 7614
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Abstract
In this study, ten strains of P. multocida isolated from chicken, cattle, buffalo and sheep had a clinical manifestation of pneumonia were identified by species-specific PCR (PM-PCR) and 460bp products were obtained. Capsular typing of P. multocida is useful for epidemiological evidence and has been assessed by conventional and genotyping assays. According to the results, only one out of the ten strains (10%) which isolated from chicken was detected as capsular type D based on acriflavine test and did not detect the capsular type A for all the ten strains (0%) by using hyaluronidase test. PCR was applied to identify the capsular types using specific primers for each type of P. multocida. The findings of this study showed that a uniform amplicon size was corresponding to 657bp, 851bp and 510bp indicating that it belongs to capsular type D, type F and type E, respectively. So, the ten strains were identified to a one strain isolated fro...
