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Bioinformatics, Identification and Cloning of β-galactosidase from Lactobacillus plantarum
ISSN: 0976 - 7614
Publisher: author
Bioinformatics, Identification and Cloning of β-galactosidase from Lactobacillus plantarum
Indexed in
Biology and Life Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 3
Article Transparency Score: 3
Article Operation Score: 3
Article Articles Score: 2
Article Accessibility Score: 2
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International Category Code (ICC):
ICC-0402
Publisher: Journal Of Advanced Laboratory Research In Biology Society..
International Journal Address (IAA):
IAA.ZONE/097644437614
eISSN
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0976 - 7614
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Abstract
Lactobacillus plantarum is a lactic acid bacterium, mostly found in fruits and vegetables. It has been used in a variety of food fermentations. It is reported that strains from this species have probiotic activity. In this study, pUC19 was chosen as a vector to clone β-galactosidase gene. To clone this gene we used EcoRI and KpnI restriction enzymes that give a DNA fragment of 5000 bp in length which contains β-galactosidase gene. The same endonuclease enzymes were used to cut the vector (pUC19). To know the length of isolated DNA fragment as well as digested pUC19, agarose gel electrophoresis was used along with Lambda HindIII and Hyperladder I respectively. QIAGEN kit was used to extract DNA fragments from the agarose gel. Then it utilised for ligation in further processes. Furthermore, the DNA fragments were transformed into host cells (E. coli) and they were spread on LB agar plates containing X-gal and IPTG to confirm the prese...
