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Bacterial DNA Induced TNF-alpha Expression in Buffaloes (Murrah) in Comparison to that of Cross Breed Cattle
ISSN: 1927 - 520X
Publisher: author
Bacterial DNA Induced TNF-alpha Expression in Buffaloes (Murrah) in Comparison to that of Cross Breed Cattle
Indexed in
Agriculture and Food Sciences
ARTICLE-FACTOR
1.3
Article Basics Score: 2
Article Transparency Score: 3
Article Operation Score: 3
Article Articles Score: 3
Article Accessibility Score: 3
SUBMIT PAPER ASK QUESTION
International Category Code (ICC):
ICC-0202
Publisher: Lifescience Global Inc.
International Journal Address (IAA):
IAA.ZONE/192710492520X
eISSN
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1927 - 520X
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Abstract
Buffaloes are generally considered to be disease resistance. But systematic studies to understand the underlying mechanism of disease resistance in buffaloes in comparison to that of cattle are scanty. Therefore, the present study was undertaken to study the immune response in terms of TNF-alpha expression in PBMCS isolated from buffaloes in comparison to that of cattle. PBMCs were isolated from blood collected from healthy buffaloes and cross breed cattle and incubated with bacterial (E. coli) DNA at different concentration for a different period of time. Total RNA was isolated and mRNA expression of TLR9 and TNF-alpha was studied. Expression of actin gen was studied as positive control. Incubation of PBMCs with bacterial DNA resulted in the expression of TLR9 in both, buffaloes and cattle. But, the expression of TNF-alpha was seen only in the case of buffaloes and the level was found to increase with the increase in bacterial DNA co...
